FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191371181
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGCG3040850.021.3425068
TGGCGCT3044950.021.21848510
ATGGCGC3060800.021.1704049
ACATGGC3085200.021.103437
TTAGCGA1643250.020.9705856
GGGAAGT3860250.020.9445721
TAGCGAG1653350.020.7766327
CGCCATG2266400.020.7623445
CCACAGG3815600.020.7336049
TCAAGCC3507800.020.558628
CACATGG2675600.020.5425536
TGGCACC2313850.020.54086910
GAGCGAC986350.020.5355177
AGCGAGA1689350.020.488168
CGTTAGC1922350.020.4865364
GAAGTTC3893500.020.463023
AAGTTCC3858050.020.4564134
GTGAGCG987050.020.444085
GTTCCAC3910700.020.4429996
AAGCCTA1993150.020.42398610
Produced by FastQC (version 0.11.5)