FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005718617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191371181
Sequences flagged as poor quality0
Sequence length98
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATT14683980.7673036202875291No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCAT10979090.5737065498906023No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA8825170.46115459777614054No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGA6831600.3569816502308151No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5674800.29653367713710244No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA4738070.24758534567438342No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGCGGCGACGACCCATTCG4574430.23903442389269675No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGGGGCGGGCGCCGGC4175460.2181864572388253No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGACGACCCATTCGAA3989900.20849011743309462No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCCCTCTCCGGCCCCGGC3956930.20676728749455753No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA3542770.18512557541252778No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT2973730.1553906907226538No Hit
GCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCG2714080.14182281709386535No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGAGTGTCCCGCGGGGCCCG2114160.11047431431172493No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGAGTGTCCCGCGGGGCCC2021840.10565018146593348No Hit
GCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTC1954860.10215017693808348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT57014850.091.0273061
AGCAGTG62816550.082.0545962
GCAGTGG69991500.073.253743
CAGTGGT72660300.070.536854
AGTGGTA74848300.068.5295
TGGTATC76944200.066.33477
GTGGTAT77120750.066.2735446
GGTATCA77540900.065.791098
GTATCAA78382700.065.4719249
GGTATCG223900.046.651968
TAGTATC945400.045.8598447
GCAGTAG968100.043.4040343
GTATCGA239300.042.6117979
CAGTAGT1018150.042.2784584
GTAGTAT1036250.041.3421446
AGTATCA1221300.036.6322338
CAACGCA77987350.035.7476512-13
ATCAACG77989550.035.68094610-11
ACGCAGA78273800.035.58864614-15
GCAGAGT78633900.035.50171316-17
Produced by FastQC (version 0.11.5)