FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162086714
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGCGT4724221129.14625747795714No Hit
ACAACTTG4241373926.167313750342302No Hit
CTCCAACA4013504924.761467494491868No Hit
TGTGTGAC2784631017.179884342648837Illumina PCR Primer Index 5 (100% over 8bp)
GNGTGCGT2005900.12375474525321059No Hit
NAGTGCGT1828530.1128118372490419No Hit
ANAACTTG1825540.11262736808891073No Hit
GAGTGCGG1784140.11007317971786386No Hit
GAGTGCGA1731510.1068261523273277No Hit
CNCCAACA1688610.10417942089935885No Hit
NCAACTTG1668610.10294551347373233No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)