FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005718631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162086714
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGGTACTCGAGGTA6162820.3802174680399777No Hit
GGATTTGACGCCCTGA4768930.29422090696465103No Hit
GTTATGCGATCATGTG4701580.29006572370885375No Hit
TGGATAGCTGGCATTT4432310.2734530110839313No Hit
TGCGCATCTTTGACCA4284630.2643418386531051No Hit
TGTACCAGATAATCGC4248400.26210661535158275No Hit
ATACCTGGAAGTCGGA4189500.2584727579831127No Hit
CGTACAAACCGCCACA4156090.2564115156286036No Hit
TGTTACTTGCTTGTGA4112280.2537086414127687No Hit
ATTTAGCCTTAGCGAG3913610.24145162200030781No Hit
TGGACGCACTGACCTA3891070.24006100833162672No Hit
AACTTGGACGGGTTCC3879370.23933917248763525No Hit
TGGGTTTGAGACCGAA3868500.23866854380180721No Hit
GCACTTCTGTGCATGT3824330.23594345925231108No Hit
GCTTTGCACAGCAGGT3611100.2227881552339941No Hit
AGGTGATCTGAAGTTA3559500.2196046740758777No Hit
TTCTAGTTGGGACCAA3552410.2191672538934931No Hit
TGGTCCTTGATGTCCA3471730.21418967133851577No Hit
TGAGAACCTGAGAGGG3446770.2126497548713339No Hit
AGAGTAATGGGGACTA3444890.21253376757332498No Hit
ACAGTTGACCGTTAGT3427030.21143188824224052No Hit
ACAAGACCTAGTTGAA3382340.20867472209967808No Hit
TCGTGAATGTGGTGAG3293560.203197407037322No Hit
GTAATCGACTACGTTG3289210.20292903217224825No Hit
GAGAGGTTGTGGCTTA3218140.19854434213528444No Hit
AGGTGATCTATGTGGG3168890.1955058450996792No Hit
CCTAAGAACAAGTTCC3121590.1925876540380725No Hit
TATGGTTCTTGGCTTA3119360.19245007336011513No Hit
CCTAAGAACTAGGTTC3077620.18987490856283262No Hit
TGGAATGCTACCCGGT3051570.18826774414095407No Hit
CTGGCCAGAGGATGGT3001360.18517001954891874No Hit
TCCATGGCTTAACGAA2994590.18475234188534415No Hit
TGGGCGTACATCCTCC2988240.18436057627770774No Hit
CGGTGTGTGTTCCATT2966130.18299649161867765No Hit
GTATGAAGACTGCGGT2809080.17330723355894548No Hit
GGTTCACCTCGATTAC2781410.1716001226355912No Hit
CGCTTCAACGAATGTA2776320.17128609319576926No Hit
GCTAGCGACCTCGATA2768710.17081659142031838No Hit
TTAAGGAGATGATGAA2624720.16193307490952033No Hit
GTTGACTGAGGACAGT2618900.16157400784866302No Hit
ATGCAAAGATAACTCG2586700.15958741689340436No Hit
CCTCATGACGGCCTGT2574190.15881560779867498No Hit
CTTGCGTACCTAACGT2500470.15426742502781565No Hit
CTTGCTGACGCTAGAG2499290.1541946244897037No Hit
AGATCCTGAAATAGCA2488730.15354312136897288No Hit
GCCTCTTGAAGGTCCC2470010.1523881840185865No Hit
ACCTCGTTGGACTCGC2452600.15131406760457863No Hit
AAAGCGGACACGTAAA2436660.1503306433863543No Hit
ACGGAATCTTTGCAGT2434410.15019182880097132No Hit
CACAGCTCTACAGGCA2421110.14937128036292968No Hit
TCTAGTGGACGGAAGT2420570.14933796486243778No Hit
CTCGAACCTCGAAAGC2409490.14865438014864069No Hit
TGGAGTTACGAAGGCT2387850.1473192923141128No Hit
CGGGACACTCGGAATG2381610.14693431319731734No Hit
AGGTAGGTGTTGGTTG2379530.14680598682505216No Hit
ACACATCGAAGATTCC2377620.14668814866590485No Hit
TCTTTAGACAACGTAA2372660.14638213962434948No Hit
GTGAAGCGAATAGGTA2361590.1456991718642652No Hit
TACACTACTTACAGAA2345350.14469723903465648No Hit
CATGGAGGATTGGGAG2342490.14452079027279188No Hit
ACACACTGACGAGGAT2328660.14366754328797116No Hit
AGAACAATGTAAGCAA2315110.1428315710071092No Hit
GGGTCACACAGGCGTA2309850.1425070533541694No Hit
ACTAGTGTGTTAGCTG2307580.1423670048613608No Hit
AGTGGTACTCGATTAC2289660.14126142380799947No Hit
CGTGAATACGTCTGGC2286450.1410633816661864No Hit
CTTTATGTGTGAACAA2240330.13821799114269168No Hit
GTTACAGTGCCTGTGG2239300.13815444491027193No Hit
CTATGTTACGCGGTAA2229650.13755908457740712No Hit
GCGGTTCGATCTTTGT2213890.13658676552601343No Hit
AAGTCTTCTCTGCGGT2194820.13541023479567857No Hit
ATGATGGCTAAATCGA2185770.1348518916855826No Hit
ACGGGAATGGAAACAT2183700.13472418226703023No Hit
ATTCCAACTAGACTTC2182310.13463842570094917No Hit
CCTTGGTACCGCCACA2170120.13388635912502983No Hit
ATGCTCCTGGCTTTGA2167990.13375494798420062No Hit
GTCCTACACCTTAAGC2165470.13359947564857166No Hit
TATTCCTGAGCCAGCT2154180.13290293490680552No Hit
TGTGACTGAACGCGAA2136010.13178193001062383No Hit
TCGCCTATGTTGCGCG2129930.13140682215323335No Hit
ATGGACTACAACTAGA2120870.13084786208942456No Hit
AAGACAAACCTGGCAC2117750.13065537253102683No Hit
TAATGGGCTGCCCAAC2104460.129835441046698No Hit
CTAAGTCGACCACTTC2100580.12959606300612644No Hit
GGTCTGGACGCTGGCT2094730.12923514508413073No Hit
TCTACTAACTGTGGGA2087660.12879895880917172No Hit
AAACATGGAGGCAGTA2087460.12878661973491548No Hit
TGACTTGGAGTCCCAG2086500.1287273921784854No Hit
TTTGGGATGTTACGAC2073090.12790005724960282No Hit
AGAGTAATGCTGTACA2064770.1273867517605422No Hit
AGGACGCCTAAGGCCA2024110.12487821796424352No Hit
AGACGCAGACGAATCT2019220.12457652759867784No Hit
CGACGACACCAGCACC2015790.12436491247518287No Hit
TACGCCTTGGACACCC2007900.12387813599577323No Hit
ACAAAGAACGTCAATC2007570.12385777652325039No Hit
TTTCTGAACGAACAGC1994890.12307547921540317No Hit
CTACGTGCTGTTTGGT1988820.12270098831172553No Hit
GCCTAAATGACCTTGA1988070.12265471678326455No Hit
CGAGGACCTCACAGTT1966080.1212980355687882No Hit
CAATCAGACTCGTCCT1952020.12043059864857276No Hit
AACTGTGACTAGGCGA1917180.11828113191313137No Hit
GTTATCACTTTATGGC1915950.11820524660645536No Hit
ATCTCTAACAACCCGC1909360.11779867410971143No Hit
CTATAGCGAGCGCACT1909150.11778571808174233No Hit
GCAATCATGATTGCCA1896270.11699108169963887No Hit
CCAGTTGCTTGTCACA1893730.1168343754565843No Hit
CTATTCAACGCACTTA1891390.116690008287786No Hit
GCCTAAATGTGCATCA1889610.11658019052690524No Hit
AGACTACTGTGCTACA1875700.11572200791238202No Hit
TTTCATCGAACAACAA1875580.11571460446782825No Hit
CGACCTTACTCGTCCT1870640.1154098293336985No Hit
TCTAGTGGAATTGGTC1869780.11535677131439656No Hit
TGTCGCTTGTTTGGGT1869200.11532098799905338No Hit
TAAACGCACCTCACTC1857310.11458743003451843No Hit
TGGCAATTGTCTTTGT1854990.11444429677314576No Hit
TTGTTAGCTCGATTCA1853910.11437766577216193No Hit
CTTGCTGACAATCCAC1847560.1139859001645255No Hit
GTCACGCGATGCACCG1846680.11393160823779795No Hit
GTCGATAACTGCGGCT1842990.11370395231776985No Hit
TTCGTCCGAGTTTGGT1841490.11361140926084787No Hit
CGAATTTCTAAATGCA1840570.11355464951926905No Hit
AACATCCGATAGCGAG1834730.1131943485509861No Hit
GTTACAGTGGAGTAAT1829950.11289944467626138No Hit
TACCACCACAAGGTGC1829940.11289882772254857No Hit
AGTGAAATGCGACAAG1829130.1128488544718107No Hit
AAGGTGCGATGATGAA1828440.11280628466562657No Hit
ATCGAAAGACATTCAG1819120.11223128380528462No Hit
TTTACCAACTAGGCGA1813900.1119092339671961No Hit
TCCGTGGGACCCGCAA1808860.11159828929593822No Hit
TACCCGGGAGGACTGA1806190.11143356265461708No Hit
AACAATGGATGCGGCT1802270.11119171679919429No Hit
TATGGAGCTCACTCAA1800040.11105413612123693No Hit
TACCTTCCTGTTTGGT1797750.1109128537210027No Hit
TGTTAACCTCTCCTAC1796980.11086534828511607No Hit
CCAGTTGCTAAGGTGC1790560.11046926400148996No Hit
TTCGATGGACGAGTTT1789510.11040448386164459No Hit
GTGAAGGACAGCAAGC1782210.10995410765129089No Hit
GCGCTACGAAAGGTGC1781320.10989919877085051No Hit
GTCGAAGGAAGGTCCC1781240.109894263141148No Hit
TTGGTCCACAGTGAGC1780110.10982454737160012No Hit
AACCTATCTTCCTACC1769880.10919340372339216No Hit
TTGGCGCGATGCTGGC1765350.10891392369148775No Hit
GCTGGACACTCCGTAA1762110.10871403068853625No Hit
GCTTCGGGATACACTG1761120.10865295227096776No Hit
AAGCCTAACAGTGAGC1752150.10809954479057426No Hit
GAGGCAATGGCAGGAT1742680.1075152896245401No Hit
GAGCCTGACCCATGTA1739810.10733822390896271No Hit
GTTGTTGGACTAACGT1737320.1071846024344722No Hit
CACATATCTATCCTTT1732070.10686070173524524No Hit
GCATCAATGAGTCTAC1730700.10677617907658983No Hit
CTTCTAAACATAGGTA1719040.10605681104744957No Hit
AGTGGTACTGAACATA1717720.10597537315735822No Hit
AGGTCGAGACATGATC1716020.10587049102617997No Hit
ATACCACGAGGTATGG1708760.10542258263067757No Hit
CAGTAGTTGAGTCTAC1707880.10536829070395No Hit
GGATACTACTGACTCA1697270.10471370281465511No Hit
CGATATGTGAGCTGAG1693640.10448974861690392No Hit
CAACACCCTGGCCAAA1693530.10448296212606296No Hit
CTTTATGTGTGAGGCA1690790.10431391680875213No Hit
TGAGTAAGAAGAGGCT1685540.10399001610952519No Hit
CGTTATGGACTGAGAG1679620.10362477951153973No Hit
GCTTCTGTGATCCTTT1677120.10347054108333642No Hit
GGACATTTGACCCGGT1676830.10345264942566484No Hit
CTCGGTTTGACCGTGC1671130.10310098580936126No Hit
GGGATCAGATTCTGGA1667290.102864075583641No Hit
CATTCCCGAATCACAC1665340.10274376960964239No Hit
GACCCACGATTTCGCC1664340.10268207423836109No Hit
GGCAACGCTACACAAA1662450.10256546998663937No Hit
GCGTAAACTCCGTGAC1659860.10240567897502074No Hit
CGAGCACTGCCGCTAG1657800.10227858651018121No Hit
GATACGTACTTGGGAG1657290.10224712187082773No Hit
TAGCAGACTGCTTACA1652920.10197751309832834No Hit
GGTTCATGAAAGGTGC1642600.10134081686670507No Hit
CGCACTTACCAGGGAT1642200.10131613871819252No Hit
CCCTGATTGTGCTCAA1637590.10103172305658563No Hit
CTAGGGCACTTGCGTA1636150.10094288172194052No Hit
GCCGTTTACGGACCAA1628300.10045857305738212No Hit
GAGGTTGGATGGAACG1623710.10017539130320083No Hit
CATCAGTGAGACTGTT1621150.10001745115272064No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)