FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005718666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343019393
Sequences flagged as poor quality0
Sequence length98
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT99517750.090.150961
AGCAGTG105671650.084.441472
GCAGTGG119617550.074.117083
CAGTGGT126447800.070.161474
AGTGGTA132387950.067.334035
GTGGTAT144912500.061.810186
TGGTATC144109050.061.595527
GGTATCA150033850.059.3854268
GTATCAA152701950.058.686169
AAGCCGT622150.047.87931
GCGGTAT440500.037.2924046
CCGTGGT882100.036.230844
AAGCGGT463900.035.499181
CGGTATC450150.035.4527
AGCGGTA476600.033.658515
AGCCGTG929050.032.0374222
CGTGGTA984200.031.9359265
GTCGTAT394900.031.6117136
GGTATCG337750.031.4019558
AGGTATC1711000.031.048397
Produced by FastQC (version 0.11.5)