FastQCFastQC Report
Fri 27 May 2022
EGAF00005718750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140285955
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT10336700.7368307112426187No Hit
ACCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT10090630.7192901099757277No Hit
TCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCT4668390.33277672023546473No Hit
ACTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG4307330.3070392898562084No Hit
TCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG4156630.2962969457633874No Hit
ACTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCT3949720.28154778573521494No Hit
ATGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGT2943550.20982499637971602No Hit
ACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA2891270.2060983225298641No Hit
ACCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC2811370.2004028129544401No Hit
TCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC2765150.19710811392345015No Hit
TCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA2541670.18117779502588124No Hit
ACCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2532000.1804884886730108No Hit
TTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGT2285470.1629150972383515No Hit
ACTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAG2144050.15283425913877122No Hit
TCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAG2135080.15219485086728746No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGAC2130410.15186195938146482No Hit
TCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2097590.149522452194163No Hit
ACCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT2080560.14830850315699814No Hit
AGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGC2051740.14625412786333458No Hit
TCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1992670.14204344262403176No Hit
AGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGAC1958540.1396105547415634No Hit
AGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA1937750.13812858172437859No Hit
TCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1923930.13714345103185846No Hit
ACTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1910220.13616616146641336No Hit
ACAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1863830.13285934432994378No Hit
TGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGC1845570.13155771723548518No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1830700.13049773942088502No Hit
ACTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1707790.12173634915911574No Hit
ACTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1647260.11742159077863497No Hit
TCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1589540.11330713755343506No Hit
TGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA1563230.11143168252302948No Hit
TCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1539230.1097208911612No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG1527450.10888117773443534No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC1443760.10291550568978913No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)