FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005718755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136087783
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGCTTGACTCGAT9124090.6704562157500942No Hit
CTGAGCGTGCTTCACC8564510.6293371683481683No Hit
GTATTCACTTGCGGCT6796330.499407797685998No Hit
CGCACACGAAAAGTAC6448590.47385517331853366No Hit
AGCCTACCTCTGGGTC6188520.4547447142995929No Hit
TGCGCATCTTCAGGTT5830220.42841612020382464No Hit
CATCTGAGAGCACTTA5701070.4189259222482888No Hit
GGACTTAACAGATTCC5600380.4115270214961177No Hit
CGTTATGGAGAGCTGA5537740.4069241101532237No Hit
TGAATGGCTTTGGCGC5244840.385401237670247No Hit
TGCAGATACACTAGTC5238310.3849214003287863No Hit
ACAAGACCTTCTGTGA5041920.37049027391386047No Hit
TGAAAGAACTGCATCA4652160.34184993666918656No Hit
ATCGGGCACATCCTAG4613490.3390083884311643No Hit
TCACATTACACCTGCG4559400.33503374803306185No Hit
CGCATCGGAGAAATCA4533180.3331070504690344No Hit
TACCGCTTGTGCACCG4491990.3300803276367578No Hit
GCTTACCACCGATTAC4478430.3290839119629129No Hit
TTGGTATCTTTCAGTG4462280.3278971779560844No Hit
TCCATTCCTGAGTAAT4449260.3269404425524369No Hit
TGGAATGCTTCTGGAT4426500.3252679926455999No Hit
AGGTTACTGAAGCTAA4381100.32193191066974763No Hit
GGAACTACTGGCATTT4363650.3206496500865181No Hit
ATACCTGGACCTTCCA4211310.30945540497195106No Hit
TCTTCAAACAGCAATA4117680.30257528701161956No Hit
ATGCTCCTGCCGATTA4089710.30051999597935986No Hit
TGCGCATCTATCTGTG4060120.2983456641365081No Hit
CATTCAACTTCGGGCT4009050.29459293932358355No Hit
AACTGTGACTCGAGAG4005020.2942968069367402No Hit
ACCTGCTACTGGCTTA3861520.28375214254170045No Hit
GGATTTGACGTTAGGG3836720.28192978939189567No Hit
TGGAACACTAGGTGAC3818240.2805718423673637No Hit
ACCTATGGATGACCTA3786700.2782542206599104No Hit
CTTGCGGGATTGGTTG3700100.271890681031963No Hit
TACTGGCCTGTTTGGT3689940.27114410409639783No Hit
TGGGAGTGAAATAGCA3666610.2694297694599081No Hit
CATTACACTCGATTAC3655520.2686148542812252No Hit
CGTGCTGGAAGGCATG3613120.2654992182509138No Hit
AGCCGGTACCAGGTGA3577220.2628612151026077No Hit
TCCTAATTGCTTCAAG3576190.26278552866130533No Hit
TGGCTAATGCTCATTT3543590.2603900160530942No Hit
AGTACGTTGAATCCTG3540650.2601739790264641No Hit
TTACTGGGAAGTCTAC3464960.25461212782046716No Hit
CCAACTCACCGGTTGC3411590.25069039444929453No Hit
CTCTGACTGCGACATT3410320.2505970723323489No Hit
TGCGCCAGACGGTTTA3353000.24638508513288074No Hit
TCTACTCCTGGGTTCC3288980.2416807686550379No Hit
AGTCCTGCTTTCCATT3288500.24164549730375134No Hit
TAGCGCGACGGACCAA3256500.2392940738846484No Hit
CGTGGAAGACTACTCC3213620.23614316650305045No Hit
TTGTACTACTTGCGTA3200070.23514748564902405No Hit
TGGAATCACAAGTTAG3188710.23431273033524253No Hit
CAGGCTTCTCAAGTTC3176130.23338832700360765No Hit
TTTAGGGACAGCAATA3174670.23328104331011107No Hit
GCTGGACACAGGACCA3160260.23222216795169628No Hit
TGGTAAGCTTGTGGGA3132030.23014777160415642No Hit
TCTACTAACACGCGAA3118620.22916237822758861No Hit
TGCGAATACAGCGCTC3115190.22891033502985347No Hit
ATCGTCCACGTTTGGT3102120.22794992552711363No Hit
AGGAGTTCTGCGCACT3100470.22782868025706615No Hit
GAAAGTAACCCTATCC3095610.22747155782528988No Hit
CTTCCTACTTTGACCA3093370.22730695818595267No Hit
TCCGGGAACCCTGCGA3074070.22588875593630622No Hit
GCATATCGATTCGTTT3055500.22452419553340802No Hit
GGTATCTGAAATGCGG3042730.22358583062522228No Hit
CGATACCCTATCCTAG3010180.22119399211610347No Hit
TCGTTCGTGAGCCTAT3002830.22065389954952827No Hit
TTGGGTAACAGCTGAG3000040.22044888482017524No Hit
TCGTATGGAAGTGAGC2995990.220151282793695No Hit
CAGAGGCCTAGGACAC2985840.2194054406779483No Hit
GTAGTGGGAACTAGGG2952500.2169555514031704No Hit
CAATCAGACCAAGTAA2923110.21479591595668807No Hit
GTCGATAACTAAAGCA2900240.2131153830318479No Hit
CAGGACCTGGCTTACA2868930.21081466218021935No Hit
CTTGACGCTACCCGGT2840630.20873512209395018No Hit
GCCCTTAACATCCTAG2831070.2080326343474932No Hit
ATGACAAGACGGTACC2824050.2075167908349275No Hit
AGCGTATACGGTGCAA2750310.20209822949353212No Hit
AAAGACTGATGACCTA2726050.2003155566139247No Hit
TCAGACCCTACCATGT2714960.19950064143524185No Hit
GCATATCGAAGTTGAA2705810.19882828130134209No Hit
ACGGGAATGTCGACAT2701240.19849246864430142No Hit
GTTCCCAACGACATAA2699070.19833301274369353No Hit
CCATGCTACTAGGTTC2632470.19343911275268552No Hit
TGCATGCCTTTCGCAA2631990.193403841401399No Hit
ACTCAGAACGATCATC2612510.19197241239502005No Hit
CGATACATGCTCATCC2603450.19130666563948653No Hit
ACGGCCTGAGCAGTTT2591050.19039548906458414No Hit
TGCGCCAGACATGCAT2562880.18832550163595507No Hit
TAATAGGGACCACAAT2547200.18717330416059463No Hit
GCTTTAGGATCATGCA2538700.18654870731489542No Hit
AGCTTGCCTTTCGCAA2536860.186413500468297No Hit
GATTCTGGAGGTTGAG2497510.18352198448261883No Hit
TTGTTTGTGTAGCGAG2487760.1828055351596109No Hit
AAACATGGAGACAAAT2482930.18245061718729005No Hit
GGGATGTGAAACGTGG2477370.18204205736822093No Hit
CGACCTACTGCTGGCT2443950.17958628953489528No Hit
TTGACGTGATTCTGAG2423790.17810489278086042No Hit
CATTGGAACTTCAGTG2413420.17734288462910738No Hit
TACCACCACAACCCGC2399830.1763442644958071No Hit
ACTCTAGGAAATCCGT2388360.1755014261640224No Hit
AAACCCTGAACGTAAA2385420.1752853891373923No Hit
GCATGGGCTTTCGGGC2383240.17512519841696592No Hit
GTCACGCGATAGGTTC2379190.17482759639048567No Hit
GGAGTTACTTGGTCCG2376900.17465932265205614No Hit
ATCTCTTGACTGCCAG2374740.1745006015712667No Hit
CCATGATGACTGCCAG2351960.1728266820247928No Hit
TAACGAAGAAGCAATA2342110.17210288450360015No Hit
CTCTATATGATGGCCC2337500.17176413256728562No Hit
TGAGGGATGTCATTGG2336410.17168403720707243No Hit
TAGACCTGAGATAGCG2333910.171500332252455No Hit
TTCGTCCGAAATCCGT2325400.17087500058693733No Hit
TAGTACCGAGCTGGCT2322730.17067880369540592No Hit
TACCACCTGGGACACA2320020.17047966752460064No Hit
GGAAGGACTTACCAAC2317340.17028273581325076No Hit
GTCGAATACTCCAGCG2294160.1685794234740381No Hit
AATGTGCTGCCTCCTA2283830.16782035460155892No Hit
CATTACACTTCCGTAA2273040.16702748401743012No Hit
TGGGCGTACTTTCTTG2267100.16659100104525915No Hit
GCACTCACTAAAGCTA2244380.16492149041769605No Hit
GCTAGATACCGATTAC2242500.16478334429182376No Hit
TGCCGGAACGGAATTA2242100.16475395149908495No Hit
ACAGACTTGGGTAGAC2240650.16464740262540686No Hit
TCCGGGATGGAGTGAC2232930.16408012172554828No Hit
ACAGTCATGTCTGTAG2231240.16395593717622692No Hit
AGGGAGTTGCATCTTT2211990.16254140902567277No Hit
CAAAGCTTGTCAATGC2209660.16237019600796937No Hit
GTCCTGACTGTTAGAA2195780.16135026609993344No Hit
ATCATCTACGAATCTC2172750.1596579760579978No Hit
GCAACTTGAGGACTGA2170360.15948235412138353No Hit
ACCAATGACGGCCATC2165740.15914286736525055No Hit
TCAAGCAACATCACAC2162510.15890552056388485No Hit
CATTAGTCTAGGTCCC2162280.15888861970806006No Hit
ACGGCCATGATCCTAG2159460.15868140051925161No Hit
ATGTCCAGAGAAGGCT2148260.1578584023225656No Hit
TCGCGTACTTCATTGG2140120.15726025899033125No Hit
CACCCATACTGGAACG2138730.15715811903556398No Hit
TGTGACTGACATTCAG2134840.15687227412617927No Hit
CCTCACATGTTGACAC2118770.1556914186778985No Hit
GTATCAGTGATTCGGT2115370.15544157993961882No Hit
ACTGTGATGTCGGGCT2085630.15325622579949003No Hit
TGCCAAATGGGGACTA2084530.15317539561945837No Hit
TCAAACTCTATGTCAC2065420.1517711549463628No Hit
ACCTATGGATTAGCTG2032740.14936976377960393No Hit
ACCTCCATGCGAACAG2019200.148374817745396No Hit
GTATTCACTCCTTCCA2001980.14710945801799122No Hit
AAATAGGTGGGTATGG1998020.14681846936987725No Hit
CTAGCTCACACGAGCA1995400.1466259465774382No Hit
AATTACCCTTCGTCCT1992210.14639153905534635No Hit
TATGGGTACCTTCACC1991810.14636214626260757No Hit
GCAAATCTGATGGCCC1991610.14634744986623818No Hit
GGGCTGACTCAGTCTA1982400.14567068081342763No Hit
ACTCTAACTTGCTCAA1979670.1454700750029854No Hit
AACCCTCACGAAGGTC1964920.14438621577074262No Hit
TCTTTCCACTGGCTTA1936970.1423323943781199No Hit
GGCGTAGCTCGGGTAA1931180.14190693370322596No Hit
GTCTGGCGAATACGCT1916100.1407988254169737No Hit
CACATGATGCCTAGGC1914950.14071432113784968No Hit
GTCCTGACTGCGGTTC1914260.14066361857037527No Hit
CTCAATTTGTTAGAAG1911030.14042627176900957No Hit
GCTACTGCTTGCTTGA1910880.1404152494717325No Hit
TACCACCACGGGTTCC1900680.13966573325689347No Hit
CCAACACCTCACAGTT1893310.1391241710506813No Hit
GTTTGAGACCTATCTT1889750.13886257519530612No Hit
CGCATCGGACCACAAT1886180.13860024452011244No Hit
GTAGATAGAATGGCCC1876150.13786322024218736No Hit
CTTCCTACTGAAGTTA1873990.1377044991613979No Hit
AATAAGGACTGAGCAG1865380.13707181929769552No Hit
GTAAGGCGATGAGCAG1863950.13696674006365434No Hit
AACCGGCACGAGAGGG1862250.13684182069451453No Hit
CTGCACGGAGTTTACG1849380.13589610758814402No Hit
TTCAAGTCTGGGTAGT1848130.13580425511083535No Hit
CCACATCACTCGGGCT1816110.13345136205209546No Hit
GGCACACACTAAGCAA1809940.13299797822409967No Hit
TCAGACCCTGACTGTT1805920.13270258065707485No Hit
CTCTGACTGGAGCCTC1804090.1325681086302949No Hit
ACGGTTAACATCCGTA1797500.1320838623699234No Hit
AGCTATCGATGACTCA1766610.12981400395067058No Hit
TACCCGGGAATCCTTT1764010.12962295079786845No Hit
GCTCCTAACGCTTCGG1762770.12953183314037822No Hit
AGTGGAGTGGGGATTG1761350.12942748872615553No Hit
CCTACGTTGACACAAA1756240.12905199579891755No Hit
TTCTAGACTCGATCGC1755000.1289608781414273No Hit
ATCTCTAACTCCAATG1742330.12802986143142622No Hit
GTGAAGGACGACTGTT1741200.12794682679193914No Hit
CACGATTGATGAGTAA1741120.1279409482333914No Hit
CTGCTTTCTGGTAGAC1718320.12626555904728054No Hit
CCACAGGTGAATGCGG1718290.12626335458782512No Hit
TGACCTCACCGGTTTA1715090.12602821224591482No Hit
CGTCAACCTTCGAGAG1714690.12599881945317604No Hit
ACCTCAGCTTCTTTGT1705390.12531543702199924No Hit
TGTTCTCCTGTGGTGA1701620.1250384099504362No Hit
GATGCGCACGTGAGCA1693870.12446892459112219No Hit
AGATCCTGACGCAACC1687730.12401774522258181No Hit
AGCTTGTGATGCATCA1672870.12292580297233588No Hit
CGCTAGTCTGCCCTTT1671600.12283248085539022No Hit
CACCCATACATAGCAA1668030.12257015018019654No Hit
TCAACAGACCGGTTGC1666470.1224555182885153No Hit
ACAGACTACGAGCTGA1660020.12198155950560235No Hit
TTGTCCCGAAGCTTGT1649410.12120191567820604No Hit
AGCCCAGTGGACTCGC1639830.12049795829211207No Hit
TAGTCCGCTTGAGTAA1639370.12046415658046247No Hit
AAAGATCGATGCATCA1617030.11882256910600124No Hit
GTCACAATGTAAGTAG1607420.11811640726045187No Hit
GTAACTGTGGGCCAAA1603830.11785260694562127No Hit
AGGTCAGGATTGCGCG1600760.11762701726135108No Hit
ATTCAGGTGGGCCTGT1584500.1164322002365194No Hit
GCGAAGCCTTGTGGGA1575930.11580245965209089No Hit
CAGATACTGGCTCCTT1563440.11488466969882226No Hit
GTGAAGGACAGTCTAC1534780.11277867609908818No Hit
TCGTAATCTTTGTTTC1524970.11205781785716945No Hit
GGGTCGACTACCAAGG1524850.11204900001934781No Hit
AATTGCCGATATCTGG1487910.10933457560992083No Hit
CGTTGCACTGTCGGTC1487300.10928975160099418No Hit
ACTCCAGCTTCCAGCG1480440.108785665205524No Hit
TAGGGTTTGGCCCAAC1480140.1087636206109699No Hit
TTGCGTCACTCTTTGT1477690.10858358975544484No Hit
AAGCATCACCCATGTA1475860.10844911772866489No Hit
TCGTAATCTTAGCTTG1469000.1079450313331947No Hit
TCAACATCTGCTACGC1459570.10725209624437779No Hit
GGTTGGAGATCTGGAT1429500.10504249305023948No Hit
GTAGCATACTGCGTGC1411550.10372349147608642No Hit
GTCCCAGGATGCACCG1409930.10360445066549435No Hit
GGTTGAACTGGTTTGT1394770.10249046382069434No Hit
GATGAATGATAATGCC1384960.10176960557877557No Hit
AACTAGCACTACGATC1379950.10140146084972228No Hit
ATCTGTACTTTCGCAA1372880.10088194323806421No Hit
AGTCGCGTGGCCCTGA1370670.10071954805818241No Hit
AGAATGTGATCATTTC1369790.10065488391415708No Hit
TCTTCAAACGCGGTTC1365980.10037491756332013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)