FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005718772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341699120
Sequences flagged as poor quality0
Sequence length98
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG53617851.5691538801738791Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC42012601.2295202867364714Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGCA11900400.348271309566147Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGCGC9220970.2698564163700509Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGC5694910.16666446199802915Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGCAGGG5094390.14908993619884067No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGCG4418130.12929884045355458Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGCGCAGG4124090.12069360904412045No Hit
AGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGCAGG4025830.11781797974779683Clontech SMARTer II A Oligonucleotide (100% over 21bp)
CAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGCAG3452970.101052938035076Clontech SMARTer II A Oligonucleotide (100% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)