FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005719956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005719956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515289901
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATGACGCGTGTAGATCT13896100.2696753802671557Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC28700.076.03183
CATTCCT38800.051.1915974
ATGCCGT32100.033.873974
GTGACTG74750.022.0129685
CGTATCA2318100.020.72219860-64
CCGTATC2282550.020.21724560-64
CGCCGTA2376050.019.91040855-59
TGACTGG76200.019.8817376
GCCGTAT2357950.019.8781655-59
TCGCCGT2411350.019.63096255-59
GTATCAT2571800.018.95652660-64
CATTAAA2775000.017.75578365-69
TCATTAA2745750.017.45329965-69
AAAACCC435600.017.25523470-74
GTCGCCG2769600.017.24925455-59
GTGCTCT69850.017.0199134
ATCATTA3016600.016.12532460-64
TGGTCGC2977650.015.91240850-54
CTCGGTG3051150.015.60071745-49
GGTCGCC3011500.015.50646755-59
Produced by FastQC (version 0.11.5)