FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005719958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005719958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505333489
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC25700.067.9774863
CATTCCT33200.050.2194024
ATGCCGT27800.016.9492454
AAAACCC225200.016.50582170-74
TACCGGG118000.015.9110775145
TCGCCGG69150.014.257013145
TCTCGGG112700.013.185961145
CTGAACC23250.013.03247510-14
GCGTCGG601450.012.462434145
CGTATCA1410300.012.42195560-64
CACTCTT30650.012.0621072
CCGTATC1426500.011.98412860-64
GCCGTAT1513500.011.26851455-59
CGCCGTA1575550.011.0271655-59
AAACCCC181350.010.96791470-74
GTATCAT1626450.010.91105860-64
CATTAAA1693550.010.76719365-69
CGGCATT177750.010.736781
TCGCCGT1629850.010.62864855-59
TCATTAA1711200.010.3909665-69
Produced by FastQC (version 0.11.5)