FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005719960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005719960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511048432
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC30400.072.00473
CATTCCT40100.050.9720654
CCAGTGA44550.029.7320961
GTGACTG82050.029.593324
CAGTGTC22150.029.226542
AGTGACT110200.025.7829133
TGACTGG85800.023.5690655
ACTGGAG111350.023.109547
CAGTTAC34400.020.933312
CCAGTGG21150.020.4143851
CAGGGAC45550.018.8432042
CAGAGAC42700.017.0346532
CAGTGGC42950.016.258082
CCAGGGA37000.016.020571
TTCCGAT191200.014.76965325-29
AAAACCC291550.014.56474270-74
CGTATCA1186850.013.91388460-64
CTGAACC27200.013.91195610-14
CCGTATC1180300.013.68769160-64
AGGGACT99250.013.5834463
Produced by FastQC (version 0.11.5)