FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005719968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005719968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512758821
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC27850.072.3185043
CATTCCT38000.049.188824
TGTACCC22400.015.539874145
CTGAACC24950.013.93988810-14
CCGTATC519450.012.49087160-64
CGTATCA544050.012.36561560-64
CGCCGTA559050.012.08281655-59
GCCGTAT566650.011.9207655-59
ATGCCGT26250.011.867784
TCGCCGT575700.011.68301855-59
CCTCAGG31650.011.227333145
CGGCATT206950.010.8554411
AAAACCC173950.010.18171870-74
TGAACCG34400.09.81558110-14
CACTCTT46650.09.6603822
GAGCGTC3466400.09.6266999
GTATCAT807250.08.75935660-64
CTGCTGA216950.08.749319
CTCGGTG782150.08.72638745-49
GGTACCC16651.369699E-98.7110405145
Produced by FastQC (version 0.11.5)