FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005719980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005719980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501829255
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGATGACGTGTAGATCT7662800.1526973551990308Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC26850.058.864193
CATTCCT33150.041.770444
AAAACCC313150.019.20209770-74
CGTATCA1462150.018.43245960-64
CCGTATC1454650.018.02211860-64
GCCGTAT1521450.017.33273555-59
CGCCGTA1551650.017.26825355-59
TCGCCGT1582700.016.94596955-59
ATGCCGT33250.016.5707114
GTATCAT1701000.015.96782460-64
CATTAAA1820500.015.2033265-69
TCATTAA1833300.014.685165-69
GTCGCCG1877450.014.51723455-59
AAACCCC250100.014.18109870-74
ATCATTA2044700.013.33763860-64
TGGTCGC2049950.013.05201350-54
AAAAACC809600.012.90776670-74
GGTCGCC2078100.012.84829255-59
CTCGGTG2118050.012.560554545-49
GCGTCGG537100.012.215383145
Produced by FastQC (version 0.11.5)