FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005719986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005719986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514036998
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTAGCTCAGTGTAGATCT7002100.13621782142615346Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC22600.091.454853
CATTCCT29300.068.066814
ATGCCGT23100.027.941414
TCTAGCC45900.022.421024145
AAAACCC348250.017.48421770-74
CGTATCA1477750.017.2126160-64
GTGCTCT70000.016.8872874
CTGAACC21400.016.86775610-14
CCGTATC1474550.016.74365460-64
CACTCTT28050.016.3129922
CGCCGTA1564550.016.06731455-59
GCCGTAT1537000.015.83651655-59
TCGCCGT1609550.015.70637555-59
GTATCAT1705700.015.07294660-64
CATTAAA1775100.014.6854965-69
CTTCCGA83000.014.5896899
TCATTAA1801500.014.0598865-69
TGAACCG26000.013.71618910-14
GTCGCCG1936400.013.26339655-59
AAAAACC836350.012.756124570-74
Produced by FastQC (version 0.11.5)