FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005719994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005719994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516437894
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC32400.085.0907443
CATTCCT38500.067.406634
CTGAACC26350.017.22424710-14
ATGCCGT23400.016.108974
CACTCTT31500.015.6714342
TGAACCG35100.012.39340610-14
CGGCATT265900.011.1408881
TGACTCC13251.8189894E-1110.942151145
CCGTATC416050.010.91111860-64
CGTATCA436400.010.68471760-64
CCTGTCC15900.010.486228145
AAAACCC120750.010.38634570-74
TCAAGCC37700.010.383434145
GTACCAC10709.943142E-810.1623955145
CGCCGTA451650.010.15950755-59
ATGACCC13001.6243575E-910.03732145
TCGCCGT467350.09.86474355-59
GCCGTAT452200.09.85860555-59
TGACCCC20100.09.737698145
CTGCTGA278300.09.5343959
Produced by FastQC (version 0.11.5)