FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005719996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005719996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504892054
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAACGAAGTGTAGATCT5414160.10723401085650677Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC25200.075.367253
CATTCCT31950.055.13434
ATGCCGT29550.048.082284
GTGCTCT58600.037.853874
CTTCCGA64000.035.791739
TCTTCCG70150.032.4473158
TCTCGGG122700.021.033758145
GTGTGCT126350.018.6563762
TGCTGTC26850.016.4688155
CTGAACC21900.015.8884410-14
AAAACCC261900.015.810670-74
TCGCCGG60150.013.739806145
GTATGCT111950.013.6054952
TATGCCT20450.013.11566453
CGTATCA1465300.012.89831460-64
CCGTATC1485750.012.44846460-64
ATGCCTT19450.012.2993134
GCGTCGG452850.011.974535145
GCCGTAT1577250.011.80179555-59
GTATCAT1655950.011.55344360-64
Produced by FastQC (version 0.11.5)