FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005719998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005719998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511371459
Sequences flagged as poor quality0
Sequence length151
%GC23

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC22800.073.442853
CATTCCT29700.052.9637034
TCTCGGG130650.023.467436145
ATGCCGT21750.021.6635254
TCGCCGG66400.017.793205145
CTGGGAT125050.016.1907121
GCGTCGG649800.015.549547145
CTGAACC20900.014.14909710-14
CTTCGAA76150.013.420969145
CAAGTGA72300.013.3445991
TAGATCC36950.012.946852145
CTTCCGA36050.012.2639599
CAAGTAG100100.012.0481331
CCGTATA34450.011.7823925145
TGAACCG25900.011.52954910-14
CAAGTTT309900.011.391881
CGGCATT167950.011.1411461
CAAGTGT89700.010.8374991
GTCGCCC19200.010.570401145
TCGACGG211850.010.469541145
Produced by FastQC (version 0.11.5)