FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509364015
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC25500.083.3303453
CATTCCT30300.065.797934
CTGAACC22100.016.9899310-14
ATGCCGT21200.013.6787474
CACTCTT28850.013.3312972
CGGTGCC14100.012.338574145
CTTCCGA42050.011.894029
CATGGCC12850.011.846472145
TGAACCG30850.011.41918610-14
GTGCTCT38250.010.9930624
CGGCATT212500.010.9485071
AGTACCC13803.8198777E-1110.505671145
AATGCCC14651.0913936E-1110.390933145
TGTCCCC13402.5283953E-1010.278309145
AGGTCCC8557.5489697E-610.173913145
TCGAACC9452.2872537E-69.972049145
CTTGGCC19250.09.79074145
TGAGACC22850.09.517172145
TCTTCCG55300.09.1762158
CTGCTGA223300.09.0565149
Produced by FastQC (version 0.11.5)