FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321344115
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG774250.07.94659043
GAGGTAA2549250.07.19649171
GCCGTCT949350.06.839676
TGCCGTC939250.06.82063065
ATGCCGT952250.06.6130244
CGTCTTC1114050.06.1541278
ACGATCC510250.06.148428145
AGGTCGA485600.06.0932392
CTCGATC1013950.05.96792657
GGTCGAG453800.05.81287863
AGGTTGC5542750.05.7531728
GGTTGCA5359150.05.7257849
CCGTCTT1186650.05.6917637
TCTCGAT1144550.05.56554376
TCGATCT1115000.05.4793968
AGATCGG6180700.05.471822145
GATTACA10941900.05.4704421
GATCGGA5978950.05.428112145
CGTCTGA3638650.05.402706145
CGGAGGT1558550.05.375155
Produced by FastQC (version 0.11.5)