FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506857576
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC33100.074.016973
CATTCCT40900.056.0024224
CTGAACC29600.014.05805310-14
CGGCATT232400.011.1903851
CACTCTT44500.010.6028552
AGTACCC18950.09.962212145
TGAACCG41600.09.79372610-14
ATGCCGT30650.09.4595954
CTGCTGA241950.09.3778419
CCGTATC452750.08.87506460-64
CGTATCA475750.08.66858660-64
CGCCGTA495700.08.34882555-59
CCTGTCC17754.441972E-98.181318145
GCCGTAT503400.08.11166155-59
TCGCCGT511950.08.07532555-59
GTTCCAC12951.3914048E-57.8496423145
CCTGCTG312950.07.71353158
ATTCCTG297600.07.64785675
TCAAGCC37050.07.447097145
CATCCGA10950.00118369127.294074145
Produced by FastQC (version 0.11.5)