FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245194762
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGG96250.038.188824145
CCACGGG3500.028.999403145
GAGCACC107800.027.909908145
GTGACGG3950.027.53108145
GTACCGG2401.6010272E-624.16617145
TCAAGGG3402.5120244E-923.4554145
CACCGGG3503.5124685E-922.785244145
TTCGTGG7750.022.45115145
TGCCTGG3905.0386006E-1022.307234145
AAAGGGG106000.021.44798975-79
TAATGGG6250.020.87957145
AACCGGG6700.020.559278145
AAGGGGG95600.020.44459275-79
ATGCCGG6450.020.232141145
GTATGCG4752.746674E-1019.841698145
AAAAGGG134300.019.44393275-79
TAGTGGG4202.8658178E-818.987703145
ACGGAGG4654.5129127E-918.709293145
GGGTCAC1950.0068460218.589361145
GATCGGG52800.018.53655145
Produced by FastQC (version 0.11.5)