FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506177771
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTGTTGGTGTAGATCT6545090.1293041768126163Illumina Single End PCR Primer 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC30850.050.5252953
CCAGTGA68400.043.335011
GTGACTG126550.032.5971074
AGTGACT173450.030.8466223
CATTCCT46450.030.4353164
CAGTGTC32950.030.1434062
TGACTGG126500.028.8847035
ACTGGAG162100.026.4767957
CAGTTAC56050.023.5408952
CATTGAC34400.022.1288092
ATGCCGT32700.021.9490814
CCAGTGG31350.019.746131
CAGAGAC73050.018.4594972
CAGGGAC70150.018.2924882
CAGTGGC59250.017.9868932
CCGATCT163450.016.89066530-34
TCCGATC165950.016.73232530-34
CTTCCGA194100.015.83675325-29
CCATTGA28200.015.4953991
GACTGGA291150.015.4136086
Produced by FastQC (version 0.11.5)