FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500389014
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC27750.077.388833
CATTCCT31550.061.575284
ATGCCGT25000.019.7169534
CACTCTT28100.015.2545952
CTGAACC22250.015.11562910-14
AAAACCC118150.012.55256270-74
CATTCCC14650.012.3701034
TCAAGCC32650.012.210519145
TGAGCCC10555.5479177E-1011.680222145
CTATGCC5300.00139970910.941295145
CGGCATT215850.010.572921
CGGTGCC14951.6370905E-1110.181992145
TGAACCG31600.010.13849410-14
CTGGGAT154950.010.1346511
CGTATCA537300.010.02767860-64
CCGTATC522450.010.00187860-64
GTCTTCC25050.09.83843145
CAACGGG58750.09.500303145
CGCCGTA571500.09.41158355-59
GCCGTAT567200.09.4011455-59
Produced by FastQC (version 0.11.5)