FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328230890
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGG59550.032.52335145
GAGCACC81550.031.139977145
CCCTGGG1603.1994932E-629.627092145
TCAAGGG4150.026.108557145
TCTAGGG2908.767529E-1025.686543145
CACCGGG2902.4636392E-823.351402145
ATGCCGG6600.022.573023145
AAAGGGG54450.022.34287675-79
CAGAGCC7300.022.263803145
CCACGGG2254.4941436E-521.068155145
AAAAGGG72350.020.59952475-79
CCGTGAG1650.003865658820.520931145
AAGGGGG48000.019.57608875-79
TTCTGGG7200.018.810852145
CGTCTGG19300.018.596428145
GATCGGG40150.017.541178145
TGATCGG21250.017.20861145
TCGCCGG4601.7654202E-716.19369145
TAATGGG5907.021299E-1016.068932145
AGGGCAA3405.831511E-515.933899145
Produced by FastQC (version 0.11.5)