FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510495141
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC28300.067.876483
CATTCCT38800.046.1494524
CGTATCA572450.013.393867560-64
CCGTATC553000.013.34842660-64
CTGAACC24550.012.93463510-14
CGCCGTA598750.012.79191155-59
GCCGTAT596250.012.75070255-59
TCGCCGT612000.012.48890155-59
AAAACCC177850.010.86018370-74
GTGCTCT42100.010.6771077
ATGCCGT25400.010.2747234
CGGCATT212950.010.0153611
CACTCTT42150.09.83293152
GTATCAT818600.09.6905260-64
AGTACCC20400.09.604895145
CTCGGTG794950.09.57433245-49
CGGTGCC18301.8189894E-129.517419145
TGAACCG33350.09.4781210-14
GAGCGTC3881150.09.4356199
TCTCGGT868150.08.93571245-49
Produced by FastQC (version 0.11.5)