FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473318234
Sequences flagged as poor quality0
Sequence length151
%GC23

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC20300.081.420323
CATTCCT24250.065.169294
ATGCCGT27450.032.483364
TAACAGG191800.028.314398145
GTGCTCT31950.024.958594
CTTCCGA36100.023.4953489
TCTTCCG34750.023.3650118
GCGTCGG445050.017.725325145
TCTCGGG83900.017.629593145
CTGAACC17450.016.53433410-14
TCGCCGG45650.016.518349145
CACTCTT31050.015.6431232
CCGTATA30550.012.578787145
CGGCATT145950.012.5511641
TGACTGG58450.011.9065365
GTGACTG68350.011.7728784
TGAACCG27600.010.45377310-14
CCCATGG6506.719785E-410.039296145
CCATTGG14901.724402E-99.245735145
CTGGGAT127750.09.1612021
Produced by FastQC (version 0.11.5)