FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428795739
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1557250.031.6393741
ATCGGAA1742200.028.4616762
TCGGAAG1868450.026.6297863
CGGAAGA2020000.024.7287714
TATGCCG947500.018.6034283
ATGCCGT1203800.014.7630044
TGCCGTC1225500.014.5429945
GCCGTCT1268650.014.0712026
CGTATGC1061300.013.1504311
CGTCTTC1538250.011.878268
AGAGCAC4512450.011.5674868
CTCGTAT892650.011.43327850-54
CCGTCTT1598600.011.4162787
TCTCGTA927150.011.08602850-54
GAGCACA4974950.010.5882979
GAAGAGC5166850.010.2345236
TCGTATG1085550.09.43366250-54
ATCTCGT1155350.09.19253150-54
GTATGCC2200800.08.3655012
CACTTCG1366650.08.11779930-34
Produced by FastQC (version 0.11.5)