FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512865384
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC33150.072.165363
CATTCCT43450.050.720554
CTGAACC26000.014.72256510-14
CACTCTT43200.012.7633162
CATGGCC14900.012.662204145
CGTATCA754150.011.92792460-64
CCGTATC742450.011.68039160-64
GCCGTAT792100.011.2823555-59
TGAACCG33100.011.21411110-14
CGCCGTA800950.011.21380955-59
TCGCCGT827850.010.85118355-59
CGGCATT238550.010.6159781
ATGCCGT28100.010.3193864
AAAACCC169650.09.87164270-74
CTGGGAT131200.09.7065391
TCTCGGG77950.09.681429145
GTATCAT994500.09.35282360-64
AACCGGG82750.08.769085145
CTGCTGA240600.08.706499
CAGGTAA125600.08.4011461
Produced by FastQC (version 0.11.5)