FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510169042
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCACTTCACGTGTAGATCT6561700.1286181531963674Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC31250.068.89733
CATTCCT38650.052.517324
ATGCCGT32150.026.3814624
CGTATCA1330950.017.74181660-64
CCGTATC1316350.017.54208860-64
AAAACCC294450.017.39717770-74
CGCCGTA1378400.017.03759455-59
TCGCCGT1401350.016.77305255-59
GCCGTAT1363800.016.74797455-59
GTATCAT1609000.014.9389860-64
CTGAACC26500.014.17045310-14
GTCGCCG1700550.013.99502355-59
CATTAAA1750600.013.90140365-69
TCATTAA1763000.013.50346265-69
CTCGGTG1763950.012.97283845-49
TCTCGGT1867250.012.65584445-49
TGGTCGC1860300.012.43875950-54
GGTCGCC1907450.012.34321355-59
GATCTCG1965950.012.0359545-49
GAGCGTC9432250.011.8036499
Produced by FastQC (version 0.11.5)