FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510304679
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC26250.082.0399253
CATTCCT32550.060.804744
ATGCCGT22950.025.9034124
AAAACCC275800.018.58879770-74
CTGAACC22050.016.90023810-14
ACTACAA40050.016.8375363
ACAAGGG112750.016.0774386
CTACAAG53400.014.1194524
CGTATCA1286150.014.0220160-64
TACAAGG131100.013.8821385
CACTACA16700.013.4795222
TCTCGGG84050.013.45429145
CCGTATC1289100.013.41408460-64
TGCTGTC19050.013.3216615
GCCGTAT1363750.013.09750955-59
AAACCCC246050.013.00505570-74
CGCCGTA1395550.012.87489955-59
CACTCTT31700.012.5989022
GTGTCCC12150.012.5290165145
TCGCCGT1444550.012.44118655-59
Produced by FastQC (version 0.11.5)