FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505044673
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC32450.067.910693
CATTCCT44050.048.0525254
CGTATCA677500.013.876286560-64
CCGTATC666950.013.64143260-64
CTGAACC29050.013.17681210-14
CGCCGTA711350.013.13411555-59
GCCGTAT709800.013.06474855-59
TCGCCGT726750.012.8398455-59
ATGCCGT30500.012.834334
AAAACCC268500.012.06875770-74
CGGCATT220150.011.1572931
ACTCGAC19700.011.066961145
CACTCTT41050.010.4349532
GTATCAT934250.010.36544360-64
TGAACCG38000.010.11148410-14
GAGCGTC4517000.09.7469899
CTCGGTG956100.09.74590945-49
GTCGCCG1024050.09.44775455-59
CTGCTGA216350.09.3824969
CATGGCC18057.2759576E-129.260278145
Produced by FastQC (version 0.11.5)