FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356659712
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC2347900.015.369129
AGAGCGT2628550.013.7335168
GATCGGA2797600.012.9913261
ATCGGAA2887250.012.6495682
TCGGAAG2984550.012.2146923
AAGAGCG3081250.011.8568927
CGTATGC430950.011.722751
CGGAAGA3130250.011.6758034
CGCCGTA839950.08.91635755-59
TCGCCGT937500.08.13548655-59
TATGCCG317500.07.7627383
GCCGTAT1014650.07.514037655-59
GAAGAGC5495850.07.1157996
GTCGCCG1090400.07.0452255-59
CGTATCA1147900.06.811130560-64
CCGTATC1102150.06.728182360-64
TGGTCGC1193100.06.478919550-54
GTGGTCG1303400.06.01628750-54
GGTCGCC1248750.05.95331655-59