FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516055489
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC31650.083.3888243
CATTCCT38500.063.8350454
CAGTGTC23400.034.7136422
CCAGTGA45000.033.2251551
GTGACTG86250.028.8307024
AGTGACT118950.027.7349363
CAGGGAC38200.026.9601942
CCAGTGG20900.025.9498481
ACTGGAG114950.023.9017417
TGACTGG96650.023.7795565
CAGAGAC32650.023.7683282
CAGTTAC36300.023.1765822
CAGTGGC45600.022.7441232
CCAGGGA30600.021.3165991
ATGCCGT26250.017.6754674
CATTGAC25600.016.7151412
CCGATCT109750.016.65985730-34
CTGAACC25950.016.59511410-14
TCCGATC112550.016.37422630-34
CTTCCGA128750.015.18039925-29
Produced by FastQC (version 0.11.5)