FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513608377
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC37950.082.7719353
CATTCCT45700.065.3491064
ATGCCGT26750.022.2202364
CATGGCC19750.016.517181145
CTGAACC33550.016.42143810-14
CACTCTT34100.013.1929712
TGAACCG40750.013.02187710-14
CGGCATT288050.011.9239791
CATCTCC32250.011.014299145
AGTTCCC9451.9751678E-710.739566145
AGTACCC19850.010.225582145
CTGCTGA305100.010.0029499
CTTGACC14701.3096724E-109.862867145
TCCTGCC38550.09.590391145
TCGGACC10701.0840868E-69.484943145
GTGCTCT46200.09.4138634
AATGCCC22500.09.343423145
CCTGTCC16309.458745E-119.339469145
GCTTACC21200.09.232481145
CTTGGCC25550.09.079242145
Produced by FastQC (version 0.11.5)