FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455338345
Sequences flagged as poor quality0
Sequence length151
%GC23

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC21250.083.923253
CATTCCT24850.067.972934
ATGCCGT24300.019.988264
CTGAACC17000.017.2285710-14
GCGTCGG410100.017.006866145
TCTCGGG77350.016.121561145
CACTCTT23600.015.0525162
TCGCCGG35800.013.365952145
CCGTATA23800.013.098769145
CGGCATT152400.012.925221
CCGTATC437400.011.401978560-64
CGTATCA472000.010.8088860-64
TGAACCG28200.010.33460210-14
CGCCGTA489700.010.29315455-59
TCGCCGT500700.010.04964455-59
GCCGTAT495700.010.03692355-59
CTGCTGA178550.09.5823919
CCATTGG13754.26553E-99.4909315145
AGATCTG127600.09.375021145
ATTCCTG189150.09.2368465
Produced by FastQC (version 0.11.5)