FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424257011
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC659000.035.6976551
TATGCCG462000.024.7130743
GAGCGTC2573050.018.9141889
AGAGCGT2889800.016.8586448
GATCGGA2995900.016.6169551
ATCGGAA3105000.016.0320322
TCGGAAG3206050.015.5630843
CGGAAGA3348000.014.8685924
AAGAGCG3408100.014.5799977
ATGCCGT708000.014.0375864
GTATGCC1710700.013.24410252
CGCCGTA913050.011.18236155-59
TGCCGTC720650.011.1657225
TCGCCGT1022550.010.108253555-59
GCCGTAT1142200.09.12553655-59
GCCGTCT803800.09.0645226
GTCGCCG1180500.08.85648855-59
GAAGAGC6319400.08.32086856
CGTATCA1311400.08.07310260-64
TGGTCGC1342350.07.88992250-54
Produced by FastQC (version 0.11.5)