FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378863302
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGG74900.030.448154145
GAGCACC95300.030.019197145
ATGCCGG7900.023.914265145
AAAGGGG97700.022.56820175-79
GATACGG14600.021.0005268
AAAAGGG135650.020.87334375-79
AAGGGGG85550.020.68783475-79
CGGTGAC4701.790795E-819.781682
CAATGGG4102.3064786E-919.748016145
CTCCAGG13150.018.984627145
CACCGGG4354.811227E-918.613073145
GCTTGAA445200.016.59311770-74
GGTGACT5802.0445259E-715.9860143
GATCGGG52900.015.56074145
ACGGAGG9550.015.543379145
CCACGGG2650.003326538515.276769145
CAAAGGG4051.590419E-514.993866145
TACGGCG15900.014.69941410-14
AAAAAGG261500.014.62371975-79
TTAAGGG8351.8189894E-1214.544947145
Produced by FastQC (version 0.11.5)