FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510083571
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTTGTGCGTGTAGATCT11482200.22510428982234365Illumina Single End PCR Primer 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC28450.079.4915853
CATTCCT33450.062.8431244
GTGACTG73650.042.2221574
TGACTGG75950.037.984715
ATGCCGT25250.037.03264
ACTGGAG87400.024.7978927
TGCTGTC20950.022.4896585
AAAACCC360100.019.00279670-74
CGTATCA2107700.018.43575360-64
CCGTATC2108300.017.7950260-64
GCCGTAT2207600.017.55692755-59
CGCCGTA2239100.017.29762555-59
CTTCCGA102600.017.08361825-29
GTATCAT2327550.016.92925360-64
CCGATCT92850.016.92538830-34
TCGCCGT2295850.016.8441555-59
CATTAAA2377650.016.71988165-69
TCATTAA2364950.016.22173765-69
TCTTCCG110900.015.80504225-29
TCCGATC91750.015.51660330-34
Produced by FastQC (version 0.11.5)