FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517581630
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATCGTGGTGTGTAGATCT11851780.22898378367872135Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC22600.076.648363
CATTCCT28450.056.556714
ATGCCGT23850.035.555844
GCAAGTG104450.022.5593386
AAAACCC460650.018.93044770-74
TGCAAGT130750.018.7960135
CGTATCA2321950.017.46803960-64
CCGTATC2327150.016.95057960-64
GTGCAAG51650.016.8393144
CAAGTGA117600.016.707587
CGCCGTA2457300.016.35747355-59
CATTAAA2540550.016.30926765-69
GCCGTAT2417050.016.27956255-59
GTATCAT2535800.016.1464260-64
TCGCCGT2510200.016.0329755-59
AGTGACT106600.015.7790969
TCATTAA2573450.015.71432765-69
AAAAACC1020450.015.56643670-74
CTGAACC19500.015.39212810-14
GGCTGCA23400.015.2121542
Produced by FastQC (version 0.11.5)