FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005720156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005720156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507971414
Sequences flagged as poor quality0
Sequence length151
%GC22

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAGCCATGGTGTAGATCT7663450.15086380431635862Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC29200.068.276143
ATGCCGT44800.061.492884
CATTCCT38000.049.2215654
TCGTGAC118100.046.039514
CATCGTG150050.045.0318572
GTGACTG120100.044.729956
TGCTGTC39550.043.0765275
TGACTGG115400.038.7616777
ACATCGA44700.038.085681
ACTGGAG135450.032.2213979
ACATCTT82000.031.5429781
ACATCGG60750.030.7898081
CATCGGG55400.030.0994132
GCCGTCG52600.029.770856
CCGTCGT52650.029.1917867
CATCGAG44200.028.540852
CTGTCGT59350.026.9957087
CATCGTT100200.026.4821532
CGGGACT47700.026.4454025
TCTTGAC46700.024.2173734
Produced by FastQC (version 0.11.5)