FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005722283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005722283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97442342
Sequences flagged as poor quality0
Sequence length80
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG1160910.11913814633067829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAC183400.020.1178931
GGGCGAT146350.019.804991
TCGCACG98050.019.40940927
TGGCGCG433450.018.5453363
GGCGCGT475500.017.9835911
CGCACGA107900.017.74095528
GGTGGCG480200.016.8221151
GGCGATC177300.016.5155962
CGCGAGT121000.016.17509313
GTCGACC232150.015.9420152
CGATCTG191850.015.5348014
GCGCGTG566900.015.16332152
GCGACAT272450.014.6605767
TGCGACA268300.014.6109976
TCGACCC265400.014.2802933
CGCGTGC608250.014.1458313
TAACACG142800.014.0347144
GTCTAAC166200.013.969471
GCGCAAT138500.013.92099246
GTGGCGC588450.013.8289212
Produced by FastQC (version 0.11.5)