FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005722317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005722317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65480669
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1156730.17665213530423765No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAG847410.12941376637431729No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGC788370.12039736490780202No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGT716160.10936968282959968No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGC693020.10583581545264908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT122100.038.454891
GGCGATC153050.030.115052
GGTGGCG383100.028.4656751
GCGATCT177550.026.1536833
TGGCGCG452250.023.8766963
CGATCTG199950.023.361684
GGCGCGT498700.021.7287834
GTGGCGC514050.021.1673642
GGTCGAC136550.018.3675751
GCGTGCC617000.017.5853737
CGCGTGC644500.016.8620766
GCGCGTG648500.016.8109995
TGCGACA292500.015.61593213
GCGACAT300050.015.3037514
CGTGCCT717800.015.2456588
AGGTCGA119750.015.0966016
AGTGCGT178150.014.62155230
TCGCGCG66450.014.17174660
GTCGACC179400.014.0766432
GCCACGA188350.013.97976419
Produced by FastQC (version 0.11.5)