FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005722361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005722361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4033390
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG1800.035.00071332
TATCGGA2700.029.182821
CGTCCGC2500.026.10085533
TCCGCAC2950.024.40909235
CCGCACA3000.023.25335736
GTCCGCA3000.022.50185434
CGTATGC10700.022.07989144
CACGTCC2700.021.66710931
AGTCACG3650.021.5749828
CGCACAT3200.021.09679637
GTCACGT3050.020.65539229
GTATGCC12450.019.5184745
TATCTCG3600.018.75224341
TCACGTC3150.018.57157730
AGTTCCG2350.017.23546234
CGTCTGA26150.017.12081116
ACACGTC27900.016.45011313
CTCGTAT14250.016.42301842
TCTCGTA14550.016.39371141
ACGTCTG28400.015.76440815
Produced by FastQC (version 0.11.5)