FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005722366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005722366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081757
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG11820.10926668373766012TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGA1050.040.7402341
CCGTATC2100.040.71951738
CGTATCT2350.037.34522239
AGTTCCG2350.037.34522234
TCCGTAT2250.037.0047537
CGCACAT2350.034.4725137
ACGTCCG2200.033.75277332
GTCACGT2850.032.36958729
TCCGCAC2450.032.14698435
CGTCCGC2500.031.50258833
TCACGTC2450.031.2256130
CACGTCC2300.030.32717931
GTCCGCA2750.030.27661334
GTTCCGT2750.030.27661335
CCGCACA2800.028.93228736
TTCCGTA3050.027.29858836
TCACGGC507.156262E-527.00097330
CTCGTAT9400.026.32951744
TCTCGTA9600.025.78217743
ATCTCGT9700.025.28558342
Produced by FastQC (version 0.11.5)