FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005722368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005722368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17303080
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA11650.023.6862811
CCGGTAC10750.023.02556634
CCCGGTA11800.021.1700133
CGGTACC11900.020.99144435
ATCGGAA14950.018.8208542
ACACGTC14600.018.17908313
ACCCGGT14150.017.81252132
TCGGAAG15750.017.7092533
CGTCTGA16250.016.19511816
CGGAAGA18350.015.4450254
TCACCCG17750.014.70697730
ACGTCTG18950.014.006330515
CACGTCT20250.012.99608214
GCACACG25500.010.67303811
CACACGT26600.010.06255212
CACCCGG28200.09.89546831
GTACCAT42300.06.91454837
GATCTCG39550.06.88746945
GGTACCA41500.06.831615436
ATGGATC35950.06.696892342
Produced by FastQC (version 0.11.5)