FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005722768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005722768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327649
Sequences flagged as poor quality0
Sequence length13-8152
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGTAGAAGCAGTGGTATCAACGCAGAGGAAAGAAGATCGGAAGAGC152181.1462366935839217Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTCGTGTAGAAGCAGTGGTATCAACGCAGAGGAAGAAGATCGGAAGAGCG143911.0839461333530171Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAAGCAGTGGTATCAACGCAGAGGAAAGAAGATCGGAAGAGCGTCGTGTA53100.3999551086168106Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAAGCAGTGGTATCAACGCAGAGGAAGAAGATCGGAAGAGCGTCGTGTAG47500.3577752854858475Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AGAAGCAGTGGTATCAACGCAGAGGAAAGAAGATCGGAAGAGCGTCGTGT25550.19244544303501906Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AAGAAGATCGGAAGAGCGTCGTGTAGAAGCAGTGGTATCAACGCAGAGGA25200.18980920408933386Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AAAGAAGATCGGAAGAGCGTCGTGTAGAAGCAGTGGTATCAACGCAGAGG22850.17210874259687614Illumina Single End PCR Primer 1 (95% over 22bp)
AGAAGCAGTGGTATCAACGCAGAGGAAGAAGATCGGAAGAGCGTCGTGTA22780.1715814948077391Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTACACGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTT22340.16826736584744914Clontech SMART CDS Primer II A (95% over 24bp)
GTCGTGTAGAAGCAGTGGTATCAACGCAGAGAAGAAGATCGGAAGAGCGT19750.14875919764937873Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTCGTGTAGAAGCAGTGGTATCAACGCAGAGGAAAAGAAGATCGGAAGAG18070.1361052507100898Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CGTCGTGTAGAAGCAGTGGTATCAACGCAGAGGAAAGAAGATCGGAAGAG15600.11750093586482573Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CGTCGTGTAGAAGCAGTGGTATCAACGCAGAGGAAGAAGATCGGAAGAGC14310.10778451232215744Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CGTGTAGAAGCAGTGGTATCAACGCAGAGGAAAGAAGATCGGAAGAGCGT14130.10642873229294791Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CGTGTAGAAGCAGTGGTATCAACGCAGAGGAAGAAGATCGGAAGAGCGTC13740.10349120889632728Clontech SMARTer II A Oligonucleotide (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)