FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005722863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005722863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921457
Sequences flagged as poor quality0
Sequence length16-8790
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACACGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTT694073.612206778501939Clontech SMART CDS Primer II A (95% over 24bp)
CTACACGACGCTCTTCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTC232871.2119448939008264Clontech SMART CDS Primer II A (95% over 24bp)
CTACGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCT184630.9608854114351766Clontech SMART CDS Primer II A (95% over 24bp)
AGTGGTATCAACGCAGAGGAAAGAAGATCGGAAGAGCGTCGTGTAGAAGC101080.5260591311697321Illumina Single End PCR Primer 1 (95% over 22bp)
AGTGGTATCAACGCAGAGGAAGAAGATCGGAAGAGCGTCGTGTAGAAGCA95000.49441647666328203Illumina Single End PCR Primer 1 (95% over 22bp)
CTACGACGCTCTTCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCTA71830.37383090019709Clontech SMART CDS Primer II A (95% over 24bp)
ACACGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCT63910.3326121791952669Clontech SMART CDS Primer II A (95% over 24bp)
CTACACGACGCTCTTCCGATCTTCTTCCTCTGCGTTGATACCACTGCTTC54980.2861370303889184Clontech SMART CDS Primer II A (95% over 24bp)
CTGCGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCT49670.2585017515354234Clontech SMART CDS Primer II A (95% over 24bp)
CTACACGACGCTCTTCCGATCTTCTTTCTCTGCGTTGATACCACTGCTTC31040.16154407826977132Clontech SMART CDS Primer II A (95% over 24bp)
ACGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCTAC30260.15748465877716752Clontech SMART CDS Primer II A (95% over 24bp)
CTGCACGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTT28730.14952195131090626Clontech SMART CDS Primer II A (95% over 24bp)
CTGCGACGCTCTTCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCTA28650.14910560059371614Clontech SMART CDS Primer II A (95% over 24bp)
CACGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCTA26700.13895705186220664Clontech SMART CDS Primer II A (95% over 24bp)
ACACGACGCTCTTCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCTA24580.12792375785666815Clontech SMART CDS Primer II A (95% over 24bp)
CTACACGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCTGCTTCT24260.1262583549879076Illumina Single End PCR Primer 1 (100% over 22bp)
CTACACGACGCTCTTCCGATCTTCTTTTCCTCTGCGTTGATACCACTGCT23230.12089783950408467Clontech SMART CDS Primer II A (95% over 23bp)
CTACACGACGCTCTTCCGATCTTCTTTCACTCTGCGTTGATACCACTGCT21390.11132177300871161Clontech SMART CDS Primer II A (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)