FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005722864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005722864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2923678
Sequences flagged as poor quality0
Sequence length17-13329
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACACGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTT1120373.832056744963023Clontech SMART CDS Primer II A (95% over 24bp)
GTAGAAGCAGTGGTATCAACGCAGAGGAAAGAAGATCGGAAGAGCGTCGT477871.6344823198724348Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTAGAAGCAGTGGTATCAACGCAGAGGAAGAAGATCGGAAGAGCGTCGTG374051.2793816555721937Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTACACGACGCTCTTCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTC345861.182962008812188Clontech SMART CDS Primer II A (95% over 24bp)
CTACGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCT341521.1681176928512647Clontech SMART CDS Primer II A (95% over 24bp)
ACACGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCT252150.8624410759324386Clontech SMART CDS Primer II A (95% over 24bp)
CTACGACGCTCTTCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCTA121690.41622230628680723Clontech SMART CDS Primer II A (95% over 24bp)
GTAGAAGCAGTGGTATCAACGCAGAGGAAAAGAAGATCGGAAGAGCGTCG102020.3489440355606876Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCTAC99570.3405641797762955Clontech SMART CDS Primer II A (95% over 24bp)
CACGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCTA86110.29452627820163507Clontech SMART CDS Primer II A (95% over 24bp)
ACACGACGCTCTTCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCTA73230.25047217922083076Clontech SMART CDS Primer II A (95% over 24bp)
GTAGAAGCAGTGGTATCAACGCAGAGAAGAAGATCGGAAGAGCGTCGTGT64150.21941540757908362Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTACACGACGCTCTTCCGATCTTCTTCCTCTGCGTTGATACCACTGCTTC46650.15955929483342557Clontech SMART CDS Primer II A (95% over 24bp)
CTACACGACGCTCTTCCGATCTTCTTTCTCTGCGTTGATACCACTGCTTC46650.15955929483342557Clontech SMART CDS Primer II A (95% over 24bp)
CTGCGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCT45320.15501023026475555Clontech SMART CDS Primer II A (95% over 24bp)
CTACACGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCTGCTTCT41540.14208130991169343Illumina Single End PCR Primer 1 (100% over 22bp)
ACACGACGCTCTTCCGATCTTCTTCCTCTGCGTTGATACCACTGCTTCTA38900.13305158776034845Clontech SMART CDS Primer II A (95% over 24bp)
AGAAGCAGTGGTATCAACGCAGAGGAAAGAAGATCGGAAGAGCGTCGTGT36690.12549261580789675Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CACGACGCTCTTCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCTAC35480.12135399315519699Clontech SMART CDS Primer II A (95% over 24bp)
CGGAAGAGCGTCGTGTAGAAGCAGTGGTATCAACGCAGAGGAAGAAGATC35370.12097775473222426Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CGGAAGAGCGTCGTGTAGAAGCAGTGGTATCAACGCAGAGGAAAGAAGAT35330.1208409407602342Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTACACGACGCTCTTCCGATCTTCTTTTCCTCTGCGTTGATACCACTGCT32330.11057989286097852Clontech SMART CDS Primer II A (95% over 23bp)
GTAGAAGCAGTGGTATCAACGCAGGAAAGAAGATCGGAAGAGCGTCGTGT32310.11051148587498351Clontech Universal Primer Mix Long (95% over 21bp)
CTACACGACGCTCTTCCGATCTTCTTTCACTCTGCGTTGATACCACTGCT31730.1085276832811274Clontech SMART CDS Primer II A (95% over 23bp)
AGAAGCAGTGGTATCAACGCAGAGGAAGAAGATCGGAAGAGCGTCGTGTA30210.10332875234550452Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTAGAAGCAGTGGTATCAACGCAGAGAAAGAAGATCGGAAGAGCGTCGTG29980.10254207200656161Clontech SMARTer II A Oligonucleotide (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)