FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005722891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005722891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2299352
Sequences flagged as poor quality0
Sequence length9-7428
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCT411371.789069268211218Clontech SMART CDS Primer II A (95% over 24bp)
ACGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCTAC140860.6126073780786935Clontech SMART CDS Primer II A (95% over 24bp)
ACACGACGCTCTTCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCTA127200.5531993361607966Clontech SMART CDS Primer II A (95% over 24bp)
GCAGTGGTATCAACGCAGAGGAAAGAAGATCGGAAGAGCGTCGTGTAGAA120760.5251914452419638Illumina Single End PCR Primer 1 (95% over 22bp)
GCAGTGGTATCAACGCAGAGGAAGAAGATCGGAAGAGCGTCGTGTAGAAG100860.43864532268221657Illumina Single End PCR Primer 1 (95% over 22bp)
AGAAGCAGTGGTATCAACGCAGAGGAAAGAAGATCGGAAGAGCGTCGTGT60200.2618128933716978Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AGAAGCAGTGGTATCAACGCAGAGGAAGAAGATCGGAAGAGCGTCGTGTA54590.23741471510234186Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGACGCTCTTCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCTACA40750.17722384393516086Clontech SMART CDS Primer II A (95% over 24bp)
GCGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCTAC33280.1447364300898688Clontech SMART CDS Primer II A (95% over 24bp)
CGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCTACA31740.13803889095710442Clontech SMART CDS Primer II A (95% over 24bp)
CTTATATGGGAGAGTTTCCCGGGCACTCACCGTGTGTAGTTGGCATCTCC26870.11685901071258338No Hit
CACGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCTA26590.11564127632480803Clontech SMART CDS Primer II A (95% over 24bp)
CAACGCAGAGGAAAGAAGATCGGAAGAGCGTCGTGTAGAAGCAGTGGTAT26450.11503240913092036Illumina Single End PCR Primer 1 (95% over 22bp)
CAACGCAGAGGAAGAAGATCGGAAGAGCGTCGTGTAGAAGCAGTGGTATC26420.114901937589373Illumina Single End PCR Primer 1 (95% over 22bp)
CTACACGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTT24280.10559496762566149Clontech SMART CDS Primer II A (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)