FastQCFastQC Report
Mon 8 Aug 2022
EGAF00005722914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005722914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3326338
Sequences flagged as poor quality0
Sequence length11-80013
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATGGGGAGAGTTTCCCGGGCACTCACCGTGTGTAGTTGGCATCTC317800.9554050129601983No Hit
CTTATATGGGAGAGTTTCCCGGGCACTCACCGTGTGTAGTTGGCATCTCC256120.7699758713636438No Hit
CTACACGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTT126580.3805385983023974Clontech SMART CDS Primer II A (95% over 24bp)
CTACGACGCTCTTCCGATCTTCTTTCCTCTGCGTTGATACCACTGCTTCT33450.10056103739307311Clontech SMART CDS Primer II A (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)